Tert-Butylhydroquinone's Effect on Oxidative Stress Indices in High Fructose Challenged Rats' Skeletal Muscle
South Asian Research Journal of Natural Products,
Antioxidant and oxidative stress effects of prooxidants are generally dose-dependent, depending on the prooxidant species and cell type. However, the cellular response to oxidant challenge is a complicated interplay of events involving cellular expression of phase II detoxification enzymes and cellular metal metabolism. The study aimed to determine tert-butylhydroquinone's effect on oxidative stress indices in high fructose-challenged rats' skeletal muscles. A total of thirty (30) experimental rats were used in this study and weighed between 150 to 183 g. The rats were grouped into four (4) groups. Group, I (control) received distilled water and standard rat pellets. Group II (disease or fructose drinking group) received 21% of fructose drinking water (w/v) and standard rat pellets. Group III (Positive control or metformin group) received 21% of fructose drinking water and oral administration of metformin (300 mg/kg body weight daily), group IV (Test group): received 21% of fructose drinking water and 1% tert-butylhydroquinone feed. The rats were fed for 49 days (7 weeks). Preparation of skeletal muscle homogenates and assay of biochemical parameters were carried out. From our results, there was a non-significant elevation/decrease for all enzymes; thus, catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione-S-transferase (GST) activity in the skeletal muscles of rats at (P>0.05) when compared to the control. MDA and GST activity expressed upregulation, while SOD and CAT expressed downregulation. TBHQ can ameliorate insulin resistance in the skeletal muscle of high fructose challenged rats, providing evidence for TBHQ's clinical use to treat T2DM.
- oxidative stress
- type 2 diabetes.
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Bokelmann I, Mahlknecht U. Valproic acid sensitizes chronic lymphocytic leukemia cells to apoptosis and restores the balance between pro-and anti-apoptotic proteins. Molecular Medicine. 2008;14:20-27.
Higashi Y, Noma K, Yoshizumi M, Kihara Y. Endothelial function and oxidative stress in cardiovascular diseases. Circular Journal. 2009;73:411-418.
Stadler K. Oxidative stress in diabetes. In: Diabetes: An Old Disease, a New Insight. Ahmad S (ed). Springer-Verlag New York, NY. 2013;272-287.
Apel K, Hirt H. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review on Plant Biology. 2004;55:373-399.
Sharma P, Jha A.B, Dubey RS, Pessarakli M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany. 2012; 217037.
Chiva-Blanch G, Badimon L. Effects of polyphenol intake on metabolic syndrome: Current evidences from human trials. Oxidative Medicine and Cellular Longevity Article. 2017;581-2401.
Eleazu C, Eleazu K, Kalu W. Management of Benign Prostatic hyperplasia: Could dietary polyphenols be an alternative to existing therapies? Frontiers in Pharmacology. 2017;8:234.
Sargazi S, Moghadam-Jafari A, Heidarpour M. Protective effect of tertbutyl hydroquinone on diazinon-induced oxidative stress in brain and heart of male rats. Zahedan Journal of Research Medicinal Science. 2016;18:1-5.
Tasset I, Perez-De La Cruz V, Elinos-Calderon D, Carrillo-Mora P, Gonzalez-Herrera IG, Luna-Lopez A, et al. Protective effect of tert-butylhydroquinone on the quinolinic-acid-induced toxicity in rat striatal slices: role of the Nrf2-antioxidant response element pathway. Neuro-signals. 2010;18(1):24–31.
Shih AY, Li P, Murphy TH. A small-molecule-inducible Nrf2-mediated antioxidant response provides effective prophylaxis against cerebral ischemia In vivo. J Neurosci. 2005;25:P10321–35.
Zhang Y, Liu FF, Bi X, Wang S, Wu X, Jiang, F. The antioxidant compound tertbutylhydroquinone activates Akt in myocardium, suppresses apoptosis and ameliorates pressure overload induced cardiac dysfunction. Scientific Reports. 2015;5:13005.
Chatterjee PK, Cuzzocrea S, Brown PA, Zacharowski K, Stewart KN, Mota-Filipe H, Thiemermann C. Tempol, a membrane-permeable radical scavenger, reduces oxidant stress-mediated renal dysfunction and injury in the rat. Kidney International. 2000;58:658-673.
Gott L. A simple method for determination of serum catalase activity and revision of reference range. Clinica Chimca Acta. 1991;196:143-151.
Habig WH, Pabst MJ, Jakoby WB. Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry. 1974;249:7130-7139.
Alptekin O, Tukel SS, Yildirim D. Immobilization and characterization of bovine liver catalase on eggshell. Journal of Serbian Chemistry Society. 2008; 73(6):609-618.
Eleazu C, Suleiman JB, Othman ZA, Zakaria Z, Nna VU, Hussain NHN, Mohamed M. Bee bread attenuates high fat diet induced renal pathology in obese rats via modulation of oxidative stress, downregulation of NF-kB mediated inflammation and Bax signalling, Archives of Physiology and Biochemistry. 2020;13-134.
Emami SR, et al. Impact of eight weeks endurance training on biochemical parameters and obesity-induced oxidative stress in high fat diet-fed rats. Journal of exercise nutrition and biochemistry. 2016;20(1):029–035.
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