Chemical Analysis of Anti-Venom Herbal Paste Use in Sri Lanka
South Asian Research Journal of Natural Products,
Background: Sri Lanka is one of the Asian countries mainly rely on herbal medicines for snake bites. A herbal paste consist of nine medicinal plants have been using for snake bites and clinically proven its efficacy. In the present study, an attempt was done to carry out chemical analysis of the paste.
Methods: Chemical analysis were carried out for the herbal paste in terms of (a) phytochemical screening (b) development of Thin Layer Chromatography fingerprint (c) antioxidant activities.
Results: Phenols, tannins, steroids, saponins and cardiac glycosides were present whereas both flavonoids and alkaloids were absent in the paste. In addition, 14 spots were observed under 254 nm and 366 nm whereas 17 spots were observed after spraying vanillin sulphate in the Thin Layer Chromatography fingerprint. Total polyphenols in the herbal paste was 94.15 ± 5.32 mg gallic acid equivalents/g of extract. Moreover, IC50 value was 628.4 ± 6.5 µg/ml for DPPH assay whereas IC50 value was 180.9 ± 2.3 µg/ml for ABTS assay.
Conclusion: Herbal paste was rich in chemical constituents and showed potent in vitro antioxidant activity.
- chemical constituents
- herbal paste
- snake bites
How to Cite
Uragoda CG. A history of medicine in Sri Lanka. 1987;275.
Liyanarachchi SK. Visha Vaidya Chintamani, Part 1, Saman Publication. Sri Lanka. 1956;49.
Silva A, et al. Victims’ response to snakebite and socio-epidemiological factors of 1018 snakebites in a tertiary care hospital in Sri Lanka. 2014. Wilderness Environ Med. 2014;25:35-40.
Karunakaran R, Thabrew MI, Thammitiyagodage GM, Galhena BP, Arawwawala LDAM. He gastroprotective effect of ethyl acetate fraction of hot water extract of Trichosanthes cucumerina Linn and its underlying mechanisms. BMC Complement Altern Med. 2017;17:312.
Dahanayake JM, Perera PK, Galappatty PG, Perera HDSM, Arawwawala LDAM. Comparative phytochemical analysis and antioxidant activities of tamalakyadi decoction with its modified dosage forms. Evid Based Complement Altern Med. 2019; Article ID 6037137.
Kulatunga WMSSK, Arawwawala LDAM. Phytochemical analysis of anti-venom traditional herbal preparation for snake-bite. South Asian Res J Nat Prod. 2019;2(4):1-6.
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999;299:152-178.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199–1200.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolonization assay. Free Radic Biol Med. 1999;26 (9-10):1231–1237.
Ranasinghe RLDS, De Silva RHSK, Arawwawala LDAM, Wijesiriwardhana, HGSG. Phytochemical variation of a polyherbal formula according to its preparation method: Qualitative and Quantitative analysis. J. Complement. Altern. Med. Res. 2020;9(1):22-28.
Amarasiri SS, Attanayake AP, Arawwawala LDAM, Jayatilaka KAPW, Mudduwa LKB. Standardized aqueous bark extract of Gmelina arborea roxb. Possesses nephroprotection against adriamycin-induced nephrotoxicity in Wistar rats. Drugs Chem. Toxicol. 2020; 1-10.
Devkota HP, Adhikari-Devkota A, Takano A, Yahara S, Basnet P. HPLC and TLC fingerprints of selected Nepalese natural medicines and medicinal plants. J Nepal Pharm Assoc. 2017;28(1):1-11.
Schofield P, Mbugua, DM, Pell AN. Analysis of condensed tannins: A review. Anim Feed Sci Tech. 2001;91:21–40.
Beekrum S, Govinden R, Padayachee T, Odhav B. Natural occurring phenols: a detoxification strategy for fumonisin B1. Food Addit Contam. 2003;20(5):490–493.
Abbas M, Saeed F, Anjum FM, et al. Natural polyphenols: an overview. Int J Food Prop. 2017;20(8):1689–1699.
Gutierrez-Grijalva EP, Ambriz-Pere DL, Leyva-Lopez NL, Castillo–Lopez RI, Heredia JB. Review: Dietary phenolic compounds, health benefits and bio-accessibility. Latin American Nutr Arch. 2016;66(2):1-24.
Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011;48(4):412–422.
Marc F, Davin A, Deglène-Benbrahim L, Ferrand C, Baccaunaud M. Studies of several analytical methods for antioxidant potential evaluation in food. Med Sci. 2004;20:458–463.
Sakong P, Khampitak T, Cha U, et al. Antioxidant activity and bioactive phytochemical contents of traditional medicinal plants North East Thailand. J Med Plant Res. 2011;5(31):6822– 6831.
Dissanayake DPA, Sivaganesh S, Tissera MHA, Handunnetti SM, Arawwawala LDAM. Comparison of antioxidant properties of Cyathula prostrate Linn and Achyranthes aspera Linn grown in Sri Lanka. Res Rev Insights. 2018;2(3):1-3.
Wakkumbura HP, Wickramaarachchi WMD, Arawwawala LDAM, Liyanage J, Rajapakse RPVJ. Assessment of the quality and evaluation of the antioxidant potential of a novel Sri Lankan Ayurvedic polyherbal formulation. Evid Based Complement Altern Med. 2020;ID: 2319315.
Abstract View: 121 times
PDF Download: 93 times