Citric Acid Production using Induced Mutant Aspergillus niger and Aspergillus awamori Isolated from Soil
South Asian Research Journal of Natural Products,
This study aimed at citric acid production using mutation induced Aspergillus niger and Aspergillus awamori isolated from soil in Keffi, Nigeria. Soil samples were collected from different location in Keffi. Aspergillus species were isolated and identify using standard microbiological method. Citric acid was produced using a batch fermentation system. Citric acid yield highest by A. awamori A1 was at 30OC with 4.23 mg/l and A. awamori A2 produced highest citric acid 30 OC with 3.93 mg/l whereas A. niger F4 yield highest at 26 OC with 5.03 mg/ml and . A. niger F5 yield the highest at 26OC with 5.75 mg/l. the yield of citric acid at different fermentation duration showed that A. awamori A1 highest after 120hrs with the 5.20 mg/l and A. awawori A2 yield highest after 120hrs with 6.00 mg/l while A. niger F4 yielded 6.09 mg/ml after 144hrs and A. niger F5 produced highest after 144hrs with 6.80 mg/ml. by A. awamori A1 yield highest 5.88 mg/ml at pH of 4.0 and A. awamori A2 produced highest also at pH of 4.0 with 5.84mg/ml. A. niger F4 yields the highest citric acid at pH of 4.5 with 4.99 mg/ml and A. niger F5 yield highest at pH of 4.5 with 7.14mg/ml. A. awamori yields highest at substrate concentrations of 125mg/l with 8.97mg/ml, A. awamori A2 yielded 8.80mg/ml in 150 mg/l substrate concentration while A. niger F4 produced highest in substrate concentration of 175 mg/l with 9.17mg/ml and A. niger F5 yielded highest in substrate concentration of 175 mg/l with 10.02 mg/ml. Strain development will play important role in citric acid and developing strains of Aspergillus species.
- Aspergillus species
- citric acid
How to Cite
Yalcin SK, Bozdemir MT, Ozbas ZY. Citric acid production by yeasts: Fermentation conditions, process optimization and strain improvement, in Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, A. Mendez-Vilas, Ed. 2010;1374–1382.
Dhilon GS, Brar SK, Verma M, Tyagi RD. Recent Advances in Citric Acid Bio-production and Recovery, Food Bioprocess Technology. 2010;6(2):30-72.
Carlos RS, Vandenberghe LPS, Rodrigues C, Pandey A. New perspectives for citric acid production and application. Food Technology and Biotechnology. 2006;44(2):141–149.
Guebel DV, Torres DNV. Optimization of the citric acid production by Aspergillus niger through a metabolic flux balance model, Electronic Journal of Biotechnology. 2001;4(1):1–2.
Akwa VL, Binbol NL, Samaila KL, Marcus ND. Geographical Perspective of Nasarawa State. Onaiv Printing and Publishing Company, Keffi. 2007;3.
Makut MD, Ifeanyi UE. Screening for citric acid producing fungi from the soil Environment of Jos North, Plateau State, Nigeria. FULafia Journal of Science & Technology. 2017;3(1):1-12.
Makut MD, Ekeleme IK. Citric acid production by Aspergillus niger and Trichoderma viride Using Hydrolysed Potato Peels Substrate Asian Journal of Advances in Agricultural Research 2018;5(1):1-7.
Cory AL, Stacy DB, Russell HJ. Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity. 2013;901697-5
Ekeleme IK, Makut MD, Owuna JE, Obiekezie SO, Muhammed FH. Citric Acid Production by Aspergillus niger and Aspergillus awamori Isolated from Soil in keffi, Nigeria. International Journal of Research and Innovation in Applied Science. 2021;6(3):44-49.
Prasad MPD, Sridevi V, Surendra babu NV, Reddy OVS, Harsha N. Studies ON fermentative production of citric acid by Aspergillus niger isolate using Sorghum Malt And Itã¢ÂÂ™S optimization. International Journal of Innovative Research in Science, Engineering and Technology. 2019;2319(8753):2347-6710.
Carlos RS, Vandenberghe LPS, Rodrigues C, Pandey A. New perspectives for citric acid production and application. Food Technology and Biotechnology. 2016; 44:2:141–149.
Asad-ur- Rehman, Sikander Ali, Ikram-ul- Haq. Temperature Optima for Citric Acid Accumulation by Aspergillus niger. Biotechnology. 2002;1:108-110.
Kishore KG, Venkata RR, Kumar D, Hanumantha RG. Production of Citric Acid by Aspergillus niger MTCC 282 in Submerged Fermentation using Colocassia antiquorum. Research Journal of Microbiology. 2008;3:150-156.
Akalin AS, Gonc S, Akbas Y. Variation in organic acids content during ripening of pickled white cheese. Journal of Dairy Sciences. 2002;85:1670-76.
Belén M, Salgado JM, Noelia R, Sandra C, Attilio C, José Manuel D. Biotechnological Production of Citric acid, Brazilian Journal of Microbiology. 2010; 41:862-875.
Singh P, Drabo S, Singh A, Chaturved S, Sharma S, Verma P. Citric acid production from different sources under submerged conditions using Aspergillus niger. International Journal of Current Microbiology and Applied Sciences. 2016; 5(5):483-492.
Ikram-ul-Haq S Ali, Qadeer MA, Iqbal J. Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based medium. Elect. J. Biotech. 2002;5:125-132.
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