Phytochemical Screening, Antimicrobial and Antioxidant Activity of Lepidium sativum Seeds Extract
South Asian Research Journal of Natural Products,
Plants play a significant role in drug discovery and development of pharmaceuticals process. In many countries, medicinal plants consider the main source of primary health care.
Aims: This study aimed to investigate the phytochemical screening, antimicrobial and antioxidant activities of Lepidium sativum seeds extracts and fractions.
Methodology: Seeds of L. sativum were collected and extracted by maceration in methanol and the extract was fractionated using hexane, chloroform, ethyl acetate and an aqueous solvent. Phytochemical screening of crude extract was performed using standard methods. The crude extracts and the fractions were tested against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. The radical scavenging (DPPH) was determined according to the standard methods.
Results: The phytochemical screening result of the crude extract showed the presence of flavonoids, alkaloids, tannins, saponins, phenols, glycosides and terpenoids. Antimicrobial activities results were showed variation in the inhibition zones from 8-20 mm; the best activity was in A. niger 20 mm at concentration 100 mg/mL of crude extract, however in S. Aureus 16 mm at the same concentration of ethyl acetate fraction. The results of free radical scavenging activity results obtained were higher at ethyl acetate fraction (76±0.02%), chloroform fraction (74±0.01%) and aqueous fraction (70±0.09%), while moderate at the crude extracts (46±0.07%) and low at hexane fraction (21±0.04%).
Conclusion: It could be concluded that the crude extracts and fractions of L. sativum had high potential as an antibacterial and antioxidant agent, which can be used for medicinal purposes.
- Lepidium sativum
- seeds extract
- phytochemical screening
- antimicrobial activity
- antioxidant activity.
How to Cite
Prashant T, Bimlesh K, Mandeep K, Gurpreet K, Harleen K. Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia. 2011;1(1):98-106.
Roshin EG, Shirly KT, Manesh K, Thankamani V. Analysis of phytoconstituents and in vitro antifungal evaluation of methanolic extract of Lepidium sativum Linn seeds. International Journal of Pharma and Bio Sciences. 2015;6(3):490 - 497.
Ramazan E, Bilal M, Ozkan S, Mahfuz E, Ali A, Ozgur E, Gulacti T. Bioassay-guided isolation, identification of compounds from Origanum rotundifolium and investigation of their antiproliferative and antioxidant activities. Pharmaceutical Biology. 2017; 55(1):1646–1653.
Neela MB, Salunkhe SS, Mali SS, Gadkari SS, Hajare AA, Gaikwad SV, Karade RS. Extraction and characterization of mucilage from Lepidium sativum. L. seeds. Der Pharmacia. 2014;6(1):65-70.
Falana H, Nofal W, Nakhleh H. A review article Lepidium sativum (Garden cress). Pharm-D Program, College of Nursing, Pharmacy and Health Professions, Birzeit University. 2014;1-8.
Sumangala SG, Nagappa GM, Mingruo G. Chemical composition of garden cress (Lepidium sativum) seeds and its fractions and use of bran as a functional ingredient. Plant Foods for Human Nutrition. 2004;59: 105–111.
Muhammad Z, Shakeel A, Luca C, Teresa M, Daniele D, Nicoletta P, Vincenzo D. Compositional study and antioxidant potential of Ipomoea hederacea Jacq and Lepidium sativum L. seed. Molecules. 2012;17:10306-10321.
Shama IYA, Shayma AMS, Warda SA. In vitro antimicrobial assessment of Lepidium sativum L. seeds extracts. Asian Journal of Medical Sciences. 2011;3(6): 261-266.
Snehal Doke, Manisha Guha. Garden cress (Lepidium sativum L.) seed - An important medicinal source: A review. Journal of Natural Product and Plant Resources. 2014;4 (1):69-80.
Nour AH, Sulieman MB, Yousf MM, Abdurahman HN, Adam MM. Bioassay-guided isolation and identificationof antifungal compounds from seeds of Swietenia macrophylla King. Australian Journal of Basic and Applied Sciences. 2016;10(17):55-62.
Alebiosu CO, Yusuf AJ. Phytochemical screening, thin-layer chromatographic studies and UV analysis of extracts of Citrullus lanatus. Journal of Pharmaceutical, Chemical and Biological Sciences. 2015;3(2):214-220.
Harborne JB. Methods of plant analysis in phytochemical methods. Springer Netherlands. 1984;1-36.
Omer Adam Omer Ishag, Ibrahim Yaagoub Erwa, Mohamed A. Diriye, Ama Almouchakara Mamane Lawane, Hamza Mohamed Ahmed, Fatimah Ali Ahmed, Shema Elnour Mergani, Alla Elamin and Awadalla B. Omer, antimicrobial potential and phytochemical screening of Eucalyptus camaldulensis and Eucalyptus microtheca leaves extracts. South Asian Research Journal of Natural Products. 2018;1(3):1-6.
Sara M. Gmaraldeen, Amira A. Magzoub, Ali M. Badri, Mohamed I. Garbi, Mahmmoud S. Saleh. Antibacterial activity of Acaci anilotica fruits extract against pathogenic bacteria. International Journal of Applied Research. 2016;2(6): 103-106.
Hiba Elawad Mohamed. Phytochemical screening and biological activity of eight medicinal plants, M.Sc. thesis, Faculty of Science - Unversity of Khartoum; 2016.
Shimada K, Fujikawan K, Yahara K, Nakamura T. Antioxidative properties of xanthanon the antioxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry. 1992;40: 8-945.
Solomon GB, Aman DB. Phytochemical screening and antimicrobial activities of crude extract of Lepidium sativium seeds grown in Ethiopia. International Journal of Pharmaceutical Sciences and Research. 2014;5(10):4182-4187.
Yohannes SG, Mussie SD, Natsnet AF. Phytochemical screening and antimicrobial potential of Lepidium sativium and Rumex nervosus in Eritrea. Journal of Advances in Medical and Pharmaceutical Sciences. 2018; 19(1):1-8.
Chatoui K, Talbaoui A, Aneb M, Bakri Y, Harhar H, Tabyaoui M. Phytochemical screening, antioxidant and antibacterial activity of Lepidium sativum seeds from Morocco. Journal of Materials and Environmental Science. Sci. 2016;7(8): 2938-2946.
Pragya Bhasin, Dinesh Bansal, Anita Punia, Anita R. Sehrawat. Antimicrobial activities of Lepidium sativum: Medicinal plant used in folklore remedies in India. Journal of Pharmacy Research. 2012;5(3): 1643-1645.
Iqbal H, Moneeb URK, Riaz U, Muhammad Z, Naeem K, Farhat AK, Zahoor U, Sajjad H. Phytochemicals screening and antimicrobial activities of selected medicinal plants of Khyber Pakhtunkhwa Pakistan. African Journal of Pharmacy and Pharmacology. 2011;5(6):746- 750.
Chairman KA, Jency M, Anita RJ, Singh Shifa VJ, Chairman K, Balasubramanian A. Antioxidative activity of different parts of the plant Lepidium sativum Linn. Biotechnology Reports. 2014;8:1–4.
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