Antibacterial Activity and Biotechnological Potential of Endophytic Bacteria Isolated from Seaweed

Sachitha Jagadheeesan

Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore-641048, Tamil Nadu, India.

Sangeetha P

Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore-641048, Tamil Nadu, India.

Yesvanth S

Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore-641048, Tamil Nadu, India.

Senthil Prabhu S *

Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore-641048, Tamil Nadu, India.

*Author to whom correspondence should be addressed.


Abstract

Seaweeds are indeed fascinating and diverse organisms found in coastal areas around the world. They play a significant role in marine ecosystems and have various uses for both humans and other marine life. They are categorized into three main groups based on coloration: red, green, and brown seaweeds. Each group has its unique characteristics and uses. Endophytic bacteria are significant contributors to the plant microbiome and are essential for the survival, development, and growth of the plant. Seaweed samples are collected and selected based on their morphology and colour. After the collection of seaweed they are cultured in TSA agar plates to obtain pure colonies. This method is usually done for the isolation of endophytic bacteria. Gram staining procedure are done to study about the morphological characteristics of the isolated bacteria. Followed by gram staining bacterial confirmation test is performed for the rapid and certain bacterial identification. Antimicrobial analysis is performed and the zone of inhibition is measured. The isolated bacteria are inoculated into the culture to produce PHB. The Sudan Black B staining method is used to detect the presence of polyhydroxybutyrate (PHB) granules in bacterial cells. Amylolytic and Proteolytic activity is also done. FTIR analysis is performed to analyze the chemical composition of substances by measuring their infrared absorption spectra. The study states that the maximum zone of inhibition of E.coli and S.aureus  is 17mm and 15mm. The PHB production using endophytic fungi from seaweed was found to produce PHB up to 900 mg of its dry cell weight.

Keywords: Seaweed, endophytic bacteria, Polyhydroxybutyrate (PHB), antimicrobial activity, enzymatic activity, FTIR analysis


How to Cite

Jagadheeesan, Sachitha, Sangeetha P, Yesvanth S, and Senthil Prabhu S. 2024. “Antibacterial Activity and Biotechnological Potential of Endophytic Bacteria Isolated from Seaweed”. South Asian Research Journal of Natural Products 7 (3):177-83. https://journalsarjnp.com/index.php/SARJNP/article/view/151.


References

John West, Hilconida P. Calumpong (Co-lead member), Georg Martin (Lead member) Sushanto Gouda, Gitishree Das, Sandeep K. Sen, HanSeung Shin, Jayanta Kumar Patra. Endophytes: A Treasure House of Bioactive Compounds of Medicinal Importance. Frontiers in Microbiology. 2016;7: 1538. Available:https://doi.org/10.3389/fmicb.2016.01538.

Ravindra N. Kharwar, Ashish Mishra, Surendra K. Gond, Andrea Stierle and Donald Stierle, Anticancer compounds derived from fungal endophytes: Their importance and future challenges. Natural Product Reports. 2011;28:1208–1228. Available:https://doi.org/10.1039/c1np00008j.

Dian Handayani, Nita Ananda, Muh. Ade Artasasta, Rustini Ruslan, Okmes Fadriyanti, Trina Ekawati Tallei. Antimicrobial activity screening of endophytic fungi extracts isolated from brown algae Padina sp. Journal of Applied Pharmaceutical Science. 2019;9(03):009- 013.

Belgacem MN, Gandini A. Monomers, Polymers and Composites from Renewable Resources. 1st ed. Elsevier Science; Amsterdam, The Netherlands; 2008.

Noorjahan A, Aiyamperumal B, Anantharaman P. Isolation and Characterisation of Seaweed Endophytic Fungi as an Efficient Phosphate Solubiizers, Biosci. Biotech. Rese. Asia. 2019;16(1):33-39.

Raviraja NS. Fungal endophytes in five medicinal plant species from Kudremukh Range, Western Ghats of India. J Basic Microbiol. 2005;4R: 230-235.

Liau CP, Ahmad MB, Shameli K, Yunus WMZW, Ibrahim NA, Zainuddin N, et al. Preparation and characterization of polyhydroxybutyrate / polycaprolactone nanocomposites. Sci World J; 2014.

DOI:10.1155/2014/572726 ;[Articleinpress}

Nair R, Kalariya T, Chanda S. Antibacterial activity of some selected indianmedicinal flora. Turk. J. Biol. 2005;29:41-47.

Yilmaz M, Soran H, Beyatli Y. Determination of poly-β-hydroxybutyrate (PHB) production by some Bacillus spp. World J Microbiol Biotechnol. 2005;21:565-6.

Brinda Devi A, Valli Nachiyar C, Kaviyarasi T. Bacillus cereus mediated synthesis of green plastics-Polyhydroxybutyrate. J Pure Appl Microbiol. 2014;8:4143-7.

Doss A, Anand, SP. Purification and characterization of extracellular amylolytic enzymes from Aspergillus species. Afr. J. Biotechnol. 2012;11(83):14941-14945.

Aretz W., Koller KP., Riess G. Proteolytic enzymes from recombinant Streptomyces lividans TK24. Biochemical Engineering Journal. 1989;65:31–6.

Bascaran V, Hardisson C, Brana AF. Regulation of extracellular protease production in Streptomyces clavuligerus. Microbiology Letters. 1990;34:208– 13

Oliveira ALLd, Felício RD, Debonsi HM, Marine natural products: chemical and biological potential of seaweeds and their endophytic fungi. Rev. Bras. Farmacogn. 2012;22:906–920.

Ahamed, Murugan: Isolation and Characterization of Marine Endophytic Fungi from Seaweeds, and Bioactivity of their Crude, Extracts, J Pure Appl Microbiol. 2019;13(3):1451-1460.

Nurbas M, Kutsal T. Production of PHB and P(HB-co-HV) Biopolymers by Using Alcaligenes Eutrophus. Iran Polymer J. 2004;13:45-51.

Godinho VM, Furbino LE, Santiago IF, Pellizzari FM, Yokoya NS, PupoD, Alves TM, Junior PA, Romanha AJ, Zani CL, Cantrell CL, Rosa CA, Rosa LH. Diversity and bioprospecting of fungal communities associated with endemic and cold adapted macroalgae in Antarctica. ISME J. 2013;7:1434–1451.

Khan MS, Zaidi A, Wani PA. Role of phosphate solubilizing microorganisms in sustainable agriculture A review. Agronomy and Sustainable Development. 2007;27: 29-43.

Loque CP, Medeiros AO, Pellizzari FM, Oliveira EC, Rosa CA, Rosa LH. Fungal community associated with marine macroalgae from Antarctica. Polar Biol, 2010;33:641–648.

Nagamani A, Kunwar IK, Manoharachary C. A HandBook of Soil Fungi, New Delhi: I K international; 2006.

Oliveira, A. L. L. d., Felício, R. D. and Debonsi, H. M., Marine natural products: Chemical and biological potential of seaweeds and their endophytic fungi. Rev. Bras. Farmacogn. 2012;22:906– 920.

Olsen SR, Sommers LE. Phosphorus. In: Page AL, Miller RH, Dennis RK (Eds). Methods of Soil Analysis. Madison: American Society of Agronomy. 1982;403±430.

Omar SA.The role of rock-phosphate-solubilizing fungi and vesicular-arbuscular-mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate. World J Microbiol Biotechnol, 1998;14(2):211–218.

Pikovskaya, R.I., Mobilization of phosphorus in soil connection with the vital activity of some microbial species. Microbiologia. 1948;17:362-370.

Yogesh C, Pathak B, Fulekar MH. PHA-production application and its bioremediation in the environment. I Res J Environ Sci. 2012;1:46-52.

Aarthi N, Ramana KV. Identification and Characterization of Polyhydroxybutyrate producing Bacillus cereus and Bacillus mycoides strains. Int J Environ Sci. 2011; 1:744-6.

Preethi R, Sasikala P, Aravind J. Microbial production of polyhydroxyalkanoate (PHA) utilizing fruzzzzit waste as a substrate. Res Biotechnol. 2012;3:61-9.

Kumalaningsih S, Hidayat N, Aini N. Optimization of Polyhydroxyalkanoates (PHA) production from liquid bean curd waste by alcaligenes latus bacteria. J Agric Food Tech. 2011;1:63-7