South Asian Research Journal of Natural Products

Phytochemical, Ethnopharmacological, and Medicinal Importance of Catharanthus roseus (Apocyanaceae): A Mini-review

Ummah Tasnim Nisat

Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh and Department of Pharmacy, University of Science and Technology, Chittagong, Bangladesh.

Nusrat Jahan

Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh and Department of Pharmacy, University of Science and Technology, Chittagong, Bangladesh.

Syeda Rubaiya Afrin

Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh.

Bakul Akter

Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh.

Ainun Nahar

Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh and Department of Pharmacy, Faculty of Life Science, Eastern University, Ashulia Model Town-1345, Dhaka, Bangladesh.

Mohammad Rashedul Islam

Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh.

Mohammed Kamrul Hossain *

Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong-4331, Bangladesh.

*Author to whom correspondence should be addressed.

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Abstract

From the ancient period, nature has always blessed human beings with a variety of medicinal plants having no or less side effects. Catharanthus roseus (Apocyanceae) is a well-known ayurvedic herb that can be found in vast quantities all around the world. The common name for C. roseus are periwinkle, pink periwinkle, Madagascar periwinkle, old maid, graveyard plant, cape periwinkle, and bright eyes. The present research has aimed to review the presence of phytochemicals, ethnopharmacological, traditional, and important medicinal information on C. roseus. This plant has traditionally been used to treat muscle discomfort, central nervous system depression, and wasp stings. Recent research has revealed that C. roseus contains more than 70 different types of alkaloids (indole alkaloids), saponins, flavonoids, carbohydrates, and chemotherapeutic agents that are effective in treating life-threatening diseases like cancers. The phytoconstituents present in C. roseus exhibited antidiabetic, anticancer, antiulcer, antioxidant, antibacterial, and other pharmacological effects in different in vitro and in vivo studies. Vinca alkaloids (vincristine and vinblastine) are one of the prominent phytoconstituents present in this plant which highly contribute to cancer chemotherapy. This mini-review will provide valid and crucial information about C. roseus to future research in natural drug discovery processes and also for clinical researchers to find vital and effective medication to treat life-threatening conditions.

Keywords: Catharanthus roseus, phytoconstituents, medicinal use, pharmacological activity, Vinblastine, vincristine

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References

Chaudhary G, Goyal S, Poonia P. Review Article Lawsonia inermis Linnaeus: A Phytopharmacological Review. Int J Pharm Sci Drug Res. 2010;2(2):91-98.

Nawaz A, Haq IU, Amin H, et al. Production and market comparison of urokinase and streptokinase as effective and cheap fibrinolytic agents for treatment of cardiovascular diseases. Acta Sci - Technol. 2022; 44(1):1-12. DOI: 10.4025/actascitechnol.v44i1.56088

Kandiah M, Chandrasekaran KN. Green Synthesis of Silver Nanoparticles Using Catharanthus roseus Flower Extracts and the Determination of Their Antioxidant, Antimicrobial, and Photocatalytic Activity. J Nanotechnol. 2021; 2021. DOI: 10.1155/2021/5512786

Jair Barrales-Cureño H, Reyes Reyes C, Vásquez García I, et al. Alkaloids of Pharmacological Importance in Catharanthus roseus. Alkaloids - Their Importance in Nature and Humane Life. IntechOpen. 2019. DOI:10.5772/intechopen.82006

Kabesh K, Senthilkumar P, Ragunathan R, Kumar RR. Phytochemical Analysis of Catharanthus roseus Plant Extract and its Antimicrobial Activity. Int J Pure Appl Biosci. 2015; 3(2):162-172.

A. Hussein R, A. El-Anssary A. Plants Secondary Metabolites: The Key Drivers of the Pharmacological Actions of Medicinal Plants, Herbal Medicine. Intechopen. 2019. DOI: 10.5772/intechopen.76139

Shukla M. Anti-Diabetic Property of Catharanthus roseusleaves and Psidium guajava Leaves. J Adv Res Ayurveda, Yoga, Unani, Sidhha Homeopath. 2020; 07 (1&2):1-5. DOI: 10.24321/2394.6547.202001

Ali H, Enshasy E, Ali H, Enshasy E. Anticancer Molecules from Catharanthus roseus. 2019; 30(3):147-156. doi:10.14499/indonesianjpharm30iss3pp147

Almagro L, Fernández-Pérez F, Pedreño MA. Indole alkaloids from Catharanthus roseus: Bioproduction and their effect on human health. Molecules. 2015; 20(2): 2973-3000.

DOI: 10.3390/molecules20022973

Pham HNT, Vuong Q Van, Bowyer MC, Scarlett CJ. Phytochemicals Derived from Catharanthus roseus and Their Health Benefits. Technologies. 2020; 8(4):80. DOI: 10.3390/technologies8040080

Van der Heijden R, Jacobs D., Snoeijer W, Hallard D, Verpoorte R. The Catharanthus alkaloids: Pharamacognosy and bio- chemistry. Curr Med Chem. 2004; 11:607-628. DOI: 10.2174/0929867043455846.

Solanki P, Gohar SK, Barua A, Baviska M. Comparative study of preliminary phytochemical extracts of Catharanthus roseus leaves. IJSDR1801003 Int J Sci Dev Res. 2018; 3(1):18-19.

Nayak BS, Anderson M, Pinto Pereira LM. Evaluation of wound-healing potential of Catharanthus roseus leaf extract in rats. Fitoterapia. 2007; 78(7-8):540-544. DOI: 10.1016/j.fitote.2007.06.008

Rinayanti A, Radji M, Mun A, Suyatna FD. Screening Angiotensin Converting Enzyme (ACE) Inhibitor Activity of Antihypertensive Medicinal Plants from Indonesia. Int J Pharm Teach Pract. 2013; 4(1):527-532.

Singh R, Pathak J, Dubey P. Catharanthus roseus : A Pharmaceutical Resource for Human diseases. Int J Res Anal Rev. 2018; 5(4):19-24.

Nisar A, Mamat AS, Hatim I, Aslam MS, Syarhabil M. Review an updated review on Catharanthus roseus: Phytochemical and pharmacological analysis. Indian Res J Pharm Sci. 2016; 9:631-653.

Aslam J, Khan SH, Siddiqui ZH, et al. Catharanthus roseus (L.) G. Don. An Important Drug: It’s Applications and Production. Int J Compr Pharm. 2010; 1 (4):1-16.

Aziz S, Saha K, Sultana N, Khan M, Nada K, Afroze M. Comparative studies of Volatile Components of the essential Oil of leaves and flowers of Catharanthus roseus growing in Bangladesh by GC-MS analysis. Indian J Pharm Biol Res. 2015; 3 (01):06-10. doi: 10.30750/ijpbr.3.1.2

Gajalakshmi S, Vijayalakshmi S, Rajeswari D V. Pharmacological activities of Catharanthus roseus: A perspective review. Int J Pharm Biol Sci. 2013; 4(2): 431-439.

de Mello JF. Plants in traditional medicine in Brazil. J Ethnopharmacol. 1980; 2(1):49-55.

DOI: 10.1016/0378-8741(80)90030-6.

Maria J, Filho B, Tavares J. Plants of the American continent with antimalarial activity. Brazilian J of Pharmacog. 2009; 19 (1A):158-192. doi: 10.1590/S0102-695X2009000100026.

Nejat N, Valdiani A, Cahill D, Tan YH, Maziah M, Abiri R. Ornamental exterior versus therapeutic interior of Madagascar periwinkle (Catharanthus roseus): The two faces of a versatile herb. Scientific World Journal. 2015; 2015:982412. DOI: 10.1155/2015/982412.

Holdsworth DK. Traditional Medicinal Plants of Rarotonga, Cook Islands Part I. Int J Crude Drug Res. 1990; 28(3):209–218.https://doi.org/10.3109/13880209009082815.

Sciences B. Diabetologia. Published online 1990:462-464.

Nisar A, Mamat AS, Hatim I, Aslam MS, Syarhabil M. Review an updated review on catharanthus roseus : Phytochemical and pharmacological analysis. Ind Res J Pharm Sci. 2016; 9: 631-653.

Chungkrang S, Sharma MS, Sharma GK, Kaushal P, Chandrul K. Phytochemical and pharmacology properties of Catharanthus rosea. Int J Res Pub Rev. 2022; 3(7):2718-2723.

Johns T. Herbal Remedies of the Luo of Siaya District, Kenya: Establishing Quantitative Criteria for Consensus 1. 1990;44(3):369-381.

Bruschi P, Morganti M, Mancini M, Signorini MA. Traditional healers and laypeople : A qualitative and quantitative approach to local knowledge on medicinal plants in Muda (Mozambique). J Ethnopharmacol. 2011; 138(2):543-563. doi:10.1016/j.jep.2011.09.055

Jordan A, Cruz D La. Use of medicinal plants for the cure of diseases and / or ailments : The case of the villager from the province of Trujillo, Peru. Manglar. 2019; 16(2): 119-124. doi:10.17268/manglar.2019.017

Hussain M, Khalid F, Noreen U, Bano A, Hussain A, Alam S, et al. An ethno-botanical study of indigenous medicinal plants and their usage in rural valleys of Swabi and Hazara region of Pakistan. Braz J Biol. 2021; 28:82:e243811. doi: 10.1590/1519-6984.243811.

Mendioro MS, Diaz MGQ, Alcantara MTB, Hilario OJ, Mateo P, Maghirang RDM. Cytological Studies of Selected Medicinal Plants : Euphorbia pulcherrima Willd . ex Klotz ., Moringa oleifera Lam ., Catharanthus roseus ( L .) Don., and Chrysanthemum indicum Linn. Philippine J Sci. 2005; 134(1):31-37.

Hsu FL, Cheng JT. Investigation in Rats of the Antihyperglycaemic Effect of Plant Extracts Used in Taiwan for the Treatment of Diabetes Mellitus. Phytother Res. 1992; 6:108-111 https://doi.org/10.1002/ptr.2650060212

Yang LL, Yen KY, Kiso Y, Hikino H. Antihepatotoxic actions of Formosan plant drugs. J Ethnopharmacol. 1987; 19(1):103-10. doi: 10.1016/0378-8741(87)90142-5.

Arnold HJ, Gulumian M. Pharmacopoeia of traditional medicine in Venda. J Ethnopharmacol. 1984; 2(1):35-74. doi: 10.1016/0378-8741(84)90086-2.

Lawal OA, Ogunwande IA, Ibirogba AE, Layode OM, Opoku AR. Chemical constituents of essential oils from Catharanthus roseus (L.) G. Don Grown in Nigeria. J Essent Oil-Bear Plants. 2015; 18(1):57-63. doi:10.1080/0972060X.2014.998720

Jeong WT, Lim HB. A UPLC-ESI-Q-TOF method for rapid and reliable identification and quantification of major indole alkaloids in Catharanthus roseus. J Chromatogr B Analyt Technol Biomed Life Sci. 2018; 30; 1080:27-36. doi: 10.1016/j.jchromb.2018.02.018

Tiong SH, Looi CY, Hazni H, Arya A, Paydar M, Wong WF,et al. Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don. Molecules. 2013; 18(8):9770-84. doi: 10.3390/molecules18089770

Renjini K, Gopakumar G, Latha M. The medicinal properties of phytochemicals in Catharanthus roseus–a review. Eur J Pharm Med Res. 2017; 4: 545-551.

Kumar S, Singh A, Kumar B, Singh B, Bahadur L, Lal M. Simultaneous quantitative determination of bioactive terpene indole alkaloids in ethanolic extracts of Catharanthus roseus (L.) G. Don by ultra-high performance liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. 2018; 151:32-41. doi: 10.1016/j.jpba.2017.12.040.

Wang CH, Wang GC, Wang Y, Zhang XQ, Huang XJ, Zhang DM, et al. Cytotoxic dimeric indole alkaloids from Catharanthus roseus. Fitoterapia. 2012; 83(4):765-9. doi: 10.1016/j.fitote.2012.03.007

Wang L, Zhang Y, He HP, Zhang Q, Li SF, Hao XJ. Three new terpenoid indole alkaloids from Catharanthus roseus. Planta Med. 2011; 77(7):754-8. doi: 10.1055/s-0030-1250569.

Shanks JV, Bhadra R, Morgan J, Rijhwani S, Vani S. Quantification of metabolites in the indole alkaloid pathways of Catharanthus roseus: implications for metabolic engineering. Biotechnol Bioeng. 1998; 58(2-3):333-8. DOI: 10.1002/(sici)1097-0290(19980420)58:2/3<333::aid-bit35>3.0.co;2-a.

Tikhomiroff C, Jolicoeur M. Screening of Catharanthus roseus secondary metabolites by high-performance liquid chromatography. J Chromatogr A. 2002; 955(1):87-93. doi: 10.1016/s0021-9673(02)00204-2.

Kaushik SHUCHI, Tomar RS, Gupta MONIKA, Mishra RK. An overview of Catharanthus roseus and medicinal properties of their metabolites against important diseases. Euro Aca Res. 2017. 5(2): 1237-1247.

Pham HNT, Vuong QV, Bowyer MC, Scarlett CJ. Pham, H.N.T., et al., Phytochemicals derived from Catharanthus roseus and their health benefits. Technologies. 2020. 8(4): p. 80. https://doi.org/10.3390/technologies8040080

Laflamme P, St-Pierre B, De Luca V. Molecular and biochemical analysis of a Madagascar periwinkle root-specific minovincinine-19-hydroxy-O-acetyltransferase. Plant Physiol. 2001. 125(1):189-198.

Jossang, A., P. Fodor, and B. Bodo, A new structural class of bisindole alkaloids from the seeds of Catharanthus roseus: vingramine and methylvingramine. J Org Chem. 1998. 63(21): p. 7162-7167.

Himes RH. Interactions of the catharanthus (Vinca) alkaloids with tubulin and microtubules. Pharmacol Ther. 1991; 51(2):257-267. doi:10.1016/0163-7258(91)90081-V

McNulty MJ, Kelada K, Paul D, Nandi S, McDonald KA. Techno-economic process modelling and Monte Carlo simulation data of uncertainty quantification in field-grown plant-based manufacturing. Data Br. 2021; 38:107317. doi:10.1016/j.dib.2021.107317

Dhyani P, Quispe C, Sharma E, et al. Anticancer potential of alkaloids: a key emphasis to colchicine, vinblastine, vincristine, vindesine, vinorelbine and vincamine. Cancer Cell Int. 2022; 22(1):1-20. doi:10.1186/s12935-022-02624-9

Thirumaran R, Prendergast GC, Gilman PB. Cytotoxic Chemotherapy in Clinical Treatment of Cancer. In Cancer immunotherapy Elsevier Inc. 2007. p. 101-116. Academic Press.. doi:10.1016/B978-012372551-6/50071-7

Starobova H, Vetter I. Pathophysiology of Chemotherapy-Induced Peripheral Neuropathy. Front Mol Neurosci. 2017; 10:174. doi: 10.3389/fnmol.2017.00174.

Lahare RP, Yadav HS, Dashahre AK, Kumar Bisen Y. An Updated Review on Phytochemical and Pharmacological Properties of Catharanthus rosea. Saudi J Med Pharm Sci. 2020; 6(12):759-766. doi:10.36348/sjmps.2020.v06i12.007

Hashim M, Arif H, Tabassum B, Rehman S, Bajaj P, Sirohi R, et al. An overview of the ameliorative efficacy of Catharanthus roseus extract against Cd2+ toxicity: implications for human health and remediation strategies. Front Public Health. 2024; 12:1327611. doi: 10.3389/fpubh.2024.

Saha A, Moitra S, Sanyal T. Anticancer and Antidiabetic Potential of Phytochemicals Derived from Catharanthus roseus: A Key Emphasis to Vinca Alkaloids. Basic Handb Indian Ethnobot Tradit Med. 2022; 1:1-19. doi:10.52756/bhietm.2022.e01.001

Rasineni K, Bellamkonda R, Singareddy SR, Desireddy S. Antihyperglycemic activity of Catharanthus roseus leaf powder in streptozotocin-induced diabetic rats. Pharmacog Res. 2010; 2(3):195-201. doi:10.4103/0974-8490.65523

Nammi S, Boini MK, Lodagala SD, Behara RBS. The juice of fresh leaves of Catharanthus roseus Linn. reduces blood glucose in normal and alloxan diabetic rabbits. BMC Complement Altern Med. 2003; 3:2-5. doi:10.1186/1472-6882-3-4

Muhammad I, Rahman N, Gul-E-nayab, Nishan U, Shah M. Antidiabetic activities of alkaloids isolated from medicinal plants. Brazilian J Pharm Sci. 2021; 57:1-14. doi:10.1590/s2175-97902020000419130

Yao XG, Chen F, Li P, Quan L, Chen J, Yu L, et al. Natural product vindoline stimulates insulin secretion and efficiently ameliorates glucose homeostasis in diabetic murine models. J Ethnopharmacol. 2013; 150(1):285-97. doi: 10.1016/j.jep.2013.08.043

Oguntibeju OO, Aboua Y, Goboza M. Vindoline—a natural product from catharanthus roseus reduces hyperlipidemia and renal pathophysiology in experimental type 2 diabetes. Biomedicines. 2019; 7(3):1-22. doi:10.3390/BIOMEDICINES7030059.

Goboza M, Aboua YG, Chegou N, Oguntibeju OO. Vindoline effectively ameliorated diabetes-induced hepatotoxicity by docking oxidative stress, inflammation and hypertriglyceridemia in type 2 diabetes-induced male Wistar rats. Biomed Pharmacother. 2019; 112 (January):108638.

DOI:10.1016/j.biopha.2019.108638.

Rahman N, Muhammad I; Gul-E-Nayab; Khan H, Aschner M, Filosa R, Daglia M. Molecular Docking of Isolated Alkaloids for Possible α-Glucosidase Inhibition. Biomolecules. 2019; 9(10):544. doi: 10.3390/biom9100544.

Tiong SH, Looi CY, Arya A, Wong WF, Hazni H, Mustafa MR, et al. Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae). Fitoterapia. 2015; 102:182-8.

DOI: 10.1016/j.fitote.2015.01.019.

Goboza M, Meyer M, Aboua YG, Oguntibeju OO. In vitro antidiabetic and antioxidant effects of different extracts of catharanthus roseus and its indole alkaloid, vindoline. Molecules. 2020; 25(23). doi:10.3390/molecules25235546.

Prabhakar PK, Doble M. Mechanism of action of natural products used in the treatment of diabetes mellitus. Chin J Integr Med. 2011; 17(8):563-574. doi:10.1007/s11655-011-0810-3.

Tiong SH, Looi CY, Hazni H, Arya A, Paydar M, Wong WF, et al. Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don. Molecules. 2013; 18(8):9770-84. doi: 10.3390/molecules18089770.

Rasool N, Rizwan K, Zubair M, Naveed KUR, Imran I, Ahmed VU. Antioxidant potential of different extracts and fractions of Catharanthus roseus shoots. Int J Phytomed. 2011; 3(1):108-114.

Jaleel CA, Gopi R, Manivannan P, Panneerselvam R. Responses of antioxidant defense system of Catharanthus roseus (L.) G. Don. to paclobutrazol treatment under salinity. Acta Physiol Plant. 2007; 29(3):205- 209. DOI:10.1007/s11738-007-0025-6.

Hassan KA, Brenda AT, Patrick V, Patrick OE. In vivo antidiarrheal activity of the ethanolic leaf extract of catharanthus roseus linn. (Apocyanaceae) in wistar rats. African J Pharm Pharmacol. 2011; 5(15):1797-1800. DOI:10.5897/AJPP11.505.

Padmaa Paarakh M, Swathi S, Taj T, Tejashwini V, Tejashwini B. Catharanthus Roseus Linn—A Review. Acta Sci Pharm Sci. 2019; 3(10):19-24. DOI:10.31080/asps.2019.03.0393.

Ruiz-Miyazawa KW, Zarpelon AC, Pinho-Ribeiro FA, Pavão-de-Souza GF, Casagrande R, Verri WA Jr. Vinpocetine reduces carrageenan-induced inflammatory hyperalgesia in mice by inhibiting oxidative stress, cytokine production and NF-κB activation in the paw and spinal cord. PLoS One. 2015; 10(3):e0118942. DOI: 10.1371/journal.pone.0118942.

Satish AM, M. K J, Gangadhar TV, Ramu R. Evaluation of Wound Healing Activity of Catharanthus Roseus Aqueous Extract in Adult Albino Rats. Int J Pharma Biol Sci. 2021;11(2):146-152. Doi:10.22376/ijpbs/lpr.2021.11.2.p146-152.

Shinde M, Chaudhari S, Gilhotra R. Evaluation of central nervous system depressant action of catharanthus roseus g. Don leaves in mice. Res J Pharm Technol. 2021; 14(3):1719-1722. DOI:10.5958/0974-360X.2021.00306.1.