Evaluation of the Hepatoprotective Potential of Methanol Seed Extract of Spondias mombin

Amah Akuma Kalu

Department of Human Physiology, Imo State University, Owerri, Imo State, Nigeria.

Eberechukwu Lolly Mbanaso *

Department of Human Physiology, Faculty of Basic Medical Sciences, Abia State University, Uturu, Abia State, Nigeria.

Kelechi Collins Onwuka

Department of Human Physiology, Faculty of Basic Medical Sciences, Abia State University, Uturu, Abia State, Nigeria.

Iheukwumere Barry Chinedu

Department of Human Physiology, Faculty of Basic Medical Sciences, Abia State University, Uturu, Abia State, Nigeria.

Ubani Clement Dimgba

Department of Human Physiology, Faculty of Basic Medical Sciences, Abia State University, Uturu, Abia State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

The liver is indisputably one of the most important organs in the body. It is saddled with the responsibility of detoxifying xenobiotics. The use of conventional synthetic drugs in restoring hepatic life and functionality is characterized by a number of pitfalls and thus, necessitates the need for plant based options. Hence, the aim of this study was to evaluate the restorative potential of the methanol seed extract of Spondias mombin on Carbon tetrachloride induced hepatic damage. Seed of Spodias mombin were dried at room temperature prior to being ground into a fine powder. The powdered plant sample was steeped in 1 L of 50% methanol for a period of 72 hours, during which the mixture was shaken twice daily. Twenty-five adult male Wistar rats were divided into five groups of five rats each. Group I was the normal control. Groups III-V were separately administered 100, 200, and 400 mg/kg of extract respectively for 7 days. On the 7th day, Groups II to V were administered with a mixture of freshly prepared CCl4 in liquid paraffin (2 ml/kg bw, 1:1 intraperitoneally) one hour after administration of the last dosing after which animals were sacrificed and blood samples collected and analyzed using standard procedures. Qualitative phytochemical screening of the extract revealed the presence of flavonoids, tannins, alkaloids, phenols, and cardiac glycoside. Oral administration of extract significantly (P≤0.05) reduced the activities of Aspartate amino transferase (AST), Alanine transaminase (ALT) and Alkaline phosphatase (ALP). The body weight of rats significantly (P≤0.05) increased in all groups except Group II, which was not administered with extract. In conclusion, methanol leaf extract of S. mombin wields the potential to restore damaged hepatocytes.

Keywords: Spondias mombin, hepatomarkers, phytochemicals, body, weight


How to Cite

Kalu, A. A., Mbanaso, E. L., Onwuka, K. C., Chinedu, I. B., & Dimgba, U. C. (2023). Evaluation of the Hepatoprotective Potential of Methanol Seed Extract of Spondias mombin. South Asian Research Journal of Natural Products, 6(3), 129–133. Retrieved from https://journalsarjnp.com/index.php/SARJNP/article/view/118

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References

Rehm J, Samokhvalov AV, Shield KD. Global burden of alcoholic liver diseases. J. Hepatolo. 2013;59:160-8.

Adeneye AA. Protective activity of the stem barks aqueous extract of Musanga cecropioides in carbon tetrachloride- and acetaminophen-induced acute hepatotoxi-city in rats. Afr. J. Tradit. Complement. Altern Med. 2009;6:131-8.

Shri JNM. Ginger: It’s role in xenobiotic metabolism. ICMR Bulletin. 2003;33(6):57-63.

Ayoka AO, Akomolafe RO, Akinsomisoye O, Sand Ukponmwan OE. Medicinal and economic value of Spondias mombin. AfrJ Biomed Res 2008;11:129 – 136.

Ademola IO, Fagbemi BO and Idowu SO. Anthelmintic activity of extract of Spondias mombin against gastrointestinal nematodes of sheep; studies In vitro and In vivo. Trop Ani Health Prod. 2005;37:223–235.

Keshinro OO. The unconventional sources of ascorbic acid in the tropics. Nutrition Report International. 1985;31:381-387.

Coates NJ, Gilpin ML, et al. SB-202742 a novel beta-lactamase inhibitor isolated from Spondias mombin. Journal of Natural Products. 1994;57:654 – 657.

Nwidu L, Elmorsy E, Yibala OI,Carter WG. Hepatoprotective effects of hydromethanolic leaf and stem extracts of Spondias mombin in carbon tetrachloride induced hepatotoxicity and oxidative stress. J Basic Clin Pharma. 2017;8: S11 S19.

National Institute of Health (NIH) revised guide for the care and use of laboratory animals NIH guide; 1996.

Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983;54:275-89.

Trease GE, Evans WC. A Textbook of pharmacognosy. Baillière Tindall, London; 2001.

Aliyu R, Adebayo AH, Gatsing D, Garba IH. The effects of ethanolic leaf extract of Commiphora africana (Burseraceae) on rat liver and kidney function. J Pharmacol Toxicol. 2006;2:373-379.

Nandy S, Datta R. Acute and subacute toxicity studies of methanolic leaves extract of Pterospermum acerifolium L wild in rodents. Int. J Pharm Life Sci. 2012; 3(3):1519-1529.