Evaluation of Antimicrobial Activities and Inhibitory Efficacy of Leave Extracts of Agaratum conyzoides and Acacia alata on Some Pathogenic Bacteria Species
Published: 2023-05-20
Page: 110-117
Issue: 2023 - Volume 6 [Issue 3]
Onuoha Udumma Nsofor
Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B.-7267, Umuahia, Abia State, Nigeria.
Alaebo Prince Ogochukwu *
Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B.-7267, Umuahia, Abia State, Nigeria.
Obike, Chiemeziem Adanma
Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B.-7267, Umuahia, Abia State, Nigeria.
Onyeabo Chimaraoke
Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B.-7267, Umuahia, Abia State, Nigeria.
Okakpu Joseph Chinemelu
Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B.-7267, Umuahia, Abia State, Nigeria.
Okolie Jennifer Chisom
Department of Biotechnology, College of Natural Sciences, Federal University of Technology, Owerri, P.M.B.-7267, Owerri, Imo State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aim: The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on some bacterial and fungal strains. In the present study, the microbial activity of extracts of leaves of Ageratum conyzoides and Acacia alata were evaluated for potential antimicrobial activity against medically important bacterial and fungal strains.
Methodology: The antimicrobial activity was determined in the extracts using agar disc diffusion method. The disc diffusion method was used to determine the antimicrobial activity of these plants on the test organisms. The fresh and ethanol extracts of Ageratum conyzoides and Acacia alata showed significant zones of inhibition greater than (5mm) on all the test organisms.
Results: The fresh and ethanol extracts of Ageratum conyzoides and Acacia alata showed significant zones of inhibition on Candida albicans (7mm) and a low inhibition on other organisms which are bacterial species.
Conclusion: The minimum inhibitory and microbial concentrations evaluated on both the fresh leaf and ethanol extracts of the plants were concentration dependent. The results of this research showed that the plants have a good antimicrobial activity and inhibitory potentials on the test organisms.
Keywords: Antifungal activity, antibacterial activity, Ageratum conyzoides, Acacia alata