Tyrosinase Inhibitory Activity of Combined Camellia sinensis (L.) Kuntze and Oryza sativa (L.) Extract Incorporated Serum

Hoang Le Son *

Department of Applied Biochemistry, School of Biotechnology, International University – Vietnam, National University – HCMC, Vietnam.

Nguyen Thi Thu Thao

Department of Applied Biochemistry, School of Biotechnology, International University – Vietnam, National University – HCMC, Vietnam.

*Author to whom correspondence should be addressed.


Abstract

Aims: The efficacy of combined Camellia sinensis (L.) Kuntze (green tea) and Oryza sativa (rice bran) extract in inhibiting the activity of tyrosinase was evaluated.

Methodology: Phytochemical analysis of the extracts was performed to detect various contituents using standard techniques, including LC-MS and GC-MS. Water- and oil-based serum were formulated with an optimum amount of combined Camellia sinensis and Oryza sativa extract. Tyrosinase assay of both formulas was determined with the presence of DOPA as oxidase activity.

Results: Anthocyanins and fatty acids were found in rice bran, whereas the green tea sample contained mainly catechins including GC, EGC, EC, EGCG, and ECG. The combination of the two extracts of equal amount (w_w) at 100 ppm achieved the optimum percentage of tyrosinase inhibitory activity (56.76% ± 0.15). Tyrosinase inhibitory activity of two serum products (water- and oil-based) containing 1% of combined Camellia sinensis and Oryza sativa extract (ratio of 5:5) were 18.7% ± 0.38 and 22.5% ± 0.41, respectively.

Conclusion: The combination of Camellia sinensis and Oryza sativa extract with the ratio of 5:5 (w_w) achieved the highest percentage of tyrosinase inhibitory activity. The catechins in green tea extract protecting against lipid oxidation of fatty acids of rice bran oil might play a key role in enhancing tyrosinase inhibitory activity.

Keywords: Camilla sinensis, Oryza sativa, LC-MS, GC-MS, tyrosinase inhibitor, serum


How to Cite

Son , H. L., & Thao , N. T. T. (2023). Tyrosinase Inhibitory Activity of Combined Camellia sinensis (L.) Kuntze and Oryza sativa (L.) Extract Incorporated Serum. South Asian Research Journal of Natural Products, 6(1), 9–15. Retrieved from https://journalsarjnp.com/index.php/SARJNP/article/view/104

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References

Vitthal VC, Atul AP, Aman BU, Anil AT. Green tea (Camellia sinensis). Chemistry, traditional, medicinal uses and its pharmacological activities - a review. Pharmacognosy Reviews. 2008;2(3):157-162.

Hanifah R, Naniek SR, Supandi, Tiara J. Tyrosinase Inhibition from Green Tea (Camellia sinensis (L.) Kuntze) gel. Indonesian Journal of Pharmaceutical Science and Technology. 2021;8(2):59-65.

No JK, Soung DY, Kim YJ, Shim KH, Jun YS, Rhee SH, et al. Inhibition of tyrosinase by green tea components. Life Sci. 1999; 65(21):241-6.

Wenhui Q, Wenya L, Dong Z, Yanli C, Anfu T, Guangming G, et al. Natural skin whitening compounds for the treatment of melanogenesis (review). Experimental and Therapeutic Medicine. 2020;20:173-185.

Reema D, Vijaya LV, S Suchiritha D. Nutritional composition of rice bran and its potentials in the development of nutraceuticals rich products. Journal of Pharmacognosy and Phytochemistry. 2021;10(2):470-473.

Neelesh KM, Radha K. Pharmacognosy of rice bran oil - a review. International Journal of Green Pharmacy. 2018;12(04)

Limtrakul (Dejkriengkraikul) P, Semmarath W, Mapoung S. Anthocyanins and proanthocyanidins in natural pigmented rice and their bioactivities. Phytochemicals in Human Health [Internet]; 2020. Available:http://dx.doi.org/10.5772/intechopen.86962

Samaneh Z, Asieh B, Mahmud THK, Munoz-Munoz J, Garcia-Molina F, Garcia-Canovas F, Ali AS. A comprehensive review on tyrosinase inhibitors. J Enzyme Inhib Med Chem. 2019;34(1):279-309.

Te-Sheng Chang. An Updated Review of Tyrosinase Inhibitors. Int J Mol Sci. 2009; 10(6):2440–2475.

Wenhui Q, Wenya L, Dong Z, Yanli C, Anfu T, Guangming G, Hua S. Natural skin-whitening compounds for the treatment of melanogenesis (review). Exp Ther Med. 2020;20(1):173-185.

Kazumasa W, Jonathan HZ, Shosuke I. Chemical and biochemical control of skin pigmentation with special emphasis on mixed melanogenesis. Pigment Cell Melanoma Res. 2021;34(4):730-747.

Meilun S, Kang L, Yefei L , Guangxin L , Jie S , Cuiqin L. Extraction optimization and purification of anthocyanins from Lycium ruthenicum Murr. and evaluation of tyrosinase inhibitory activity of the anthocyanins. Journal of Food Science. 2020;85(3):696-706.

Kubota M, Hosoya T, Fukumoto S, Miyagi T, Kumazawa S. Anti-melanogenic compounds in Rubus croceacanthus. Journal of Berry Research. 2014;4:127-135.

Jeung-Hyun K, Ikjae L, Seok-Kweon Y, Han-Uk K, Byung-Hyun P, Jin-Woo P. Saponified sunflower and safflower oils inhibit melanogenesis in B16 melanoma cells. Mol Med Rep. 2010;3(2):281-5.

Stefania B, Emanuela C, Mauro P. Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Research. 2003;16(2):101-110.

Ando H, Ryu A, Hashimoto A, Oka M, Ichashi M. Linoleic and α-linoleic acid lightens ultraviolet-induced hyperpigmenta-tion of the skin. Arch Dermatol Res. 1998;290:375-381.

Ando H, Funasaka Y, Oka M, Ohashi A, Furumura M, Matsunaga J, et al. Possible involvement of proteolytic degradation of tyrosinase in the regulatory effect of fatty acids on melanogenesis. J Lipid Res. 1999;40:1312-1316.

Chang-Soon K, Jae HP. Antioxidant Activity of Green Tea Extract in Soybean and Rice Bran Oils. Nutraceuticals and Food. 2002;7(2):151-156.

Haifeng T, Fengchao C, Haijuan L, Qingrong H, Yunqi L. Understanding the inhibitory mechanism of tea polyphenols against tyrosinase using fluorescence spectroscopy, cyclic voltammetry, oximetry, and molecular simulations. The Royal Society of Chemistry. 2018;8:8310-8318.