South Asian Research Journal of Natural Products <p style="text-align: justify;"><strong>South Asian Research Journal of Natural&nbsp;Products</strong>&nbsp;aims to publish&nbsp;high-quality&nbsp;papers (<a href="/index.php/SARJNP/general-guideline-for-authors">Click here for Types of paper</a>) covering all aspects of research&nbsp;of naturally occurring compounds or the biology of living systems from which they are obtained.</p> South Asian Research Journal of Natural Products en-US South Asian Research Journal of Natural Products Influence of Retention Time on the Optimization of Biogas Production from Water Hyacinth and Cow Dung Blend <p>The aim of this study was to evaluate the influence of retention time on the production of biogas from a blend of cow dung and water hyacinth. The study was designed to include three production trials. These were the Cow dung Production Trial (CPT), Water Hyacinth Production Trial (WPT) and Cow dung-Water hyacinth Production Trial (CWPT). For the CPT, 0.5 kg of cow dung was dissolved in 7 litres of water to form slurry which was sieved before being introduced into the digester alone, while for the WPT, chopped water hyacinth was introduced into the digester alone and for the CWPT, cow dung slurry alongside chopped water hyacinth was introduced into the digester. The three production trials were performed at the temperature of 35ºC and pH of 6.8 and lasted for 40 days in ranges of 0-10, 11-20, 21-30 and 31-40. Results obtained from the study showed a uniform trend in the production of biogas for the three different production trials. Highest level of biogas production was recorded at day 11-21 thus, CPT (53.7 ml), WPT (35.67 ml) and CWBPT (89.37 ml). This was followed the values recorded on day 31-40 as follows; CPT (38.1 ml), WPT (22.1 ml) and CWBPT (60.02 ml). However, this was elevated compared to the values obtained for day 0-10. In conclusion, this study has established that biogas production from cow dung, water hyacinth as well as their combination is most efficiently achieved between 11<sup>th</sup>-21<sup>st</sup> day of production.</p> N. A. Osaribie O. Ewa M. R. Karimah Ayuba Victoria ##submission.copyrightStatement## 2019-12-17 2019-12-17 1 5 Analysis of Soil Quality in a Deltaic Hydrocarbon Polluted Environment Niger Delta Nigeria <p><strong>Aim:</strong> The study was aimed to ascertain the damage done by an oil spill on the soils of Ogbia area in Bayelsa State, Niger Delta, Nigeria.</p> <p><strong>Study Design:</strong> The study adopted the experimental research design which entailed the use of field measurements and a control site. Three communities which are Elebele, Imiringi and Otuasega were used for the study. At each of the sample locations, three random spots were augered at two depth-levels (Top Sample (T), 0 – 15 cm; Bottom Sample (B), 15- 30 cm), with the aid of an auger to collect the samples for laboratory analysis. The parameters of interest to the study are TPH, THC, Organic Matter, THF, THB, pH, sand, silt clay and soil texture and these were analysed using standard techniques as recommended by DPR.</p> <p><strong>Results:</strong> The study revealed that there is a noticeable effect of oil exploitation activities on soil quality within the study area, In the case of THC, the result revealed that the level of THC in the sampled communities was higher than that of the non spill site, hence the presence of hydrocarbon which has caused the pollution of the soil. Organic matter content of the soil also reveals that the non-polluted site has more organic content than the sampled communities.</p> <p><strong>Conclusion:</strong> The study revealed that there is a statistically significant difference in soil quality of the selected communities and that of the non-spilt site. The study, therefore, recommended complete and total remediation of the soils in the area, as this will enhance the soil for increased food production.</p> Chukwu Okeah, G. O. Ushie, Lawrence Oyegun, Charles Uwadiae ##submission.copyrightStatement## 2019-12-31 2019-12-31 1 9 Phytochemical Analysis of an Anti-venom Traditional Herbal Preparation for Snake-bite <p><strong>Objective: </strong>Snake-bite is one of the important public health problems of tropical countries including Sri Lanka. The risk of snake-bites is higher in rural areas of the country and people mainly rely on herbal medicines. Antiserum is the only therapeutic agent in Western medicine available throughout the world. A major drawback of serum therapy is its higher cost and also serum sickness is a possible side effect of serum therapy that results in inflammation of tissues and other symptoms. In the present study, an attempt was taken to prepare a traditional herbal paste which used to treat snake-bites and carry out chemical analysis.</p> <p><strong>Methods:</strong> Chemical analysis carried out by investigation of its(a) phytochemical constituents (b) total phenol and flavonoid contents and (c) development of Thin Layer Chromatography (TLC) fingerprints.</p> <p><strong>Results:</strong> Results revealed that phenols, flavonoids, tannins and saponins were abundant in the herbal paste whereas coumarins, terpenoids and alkaloids were absent.&nbsp; Further high amounts of total phenols (120.30±0.83 mg gallic acid equivalents /g) and flavonoids (69.76±1.62 quercetin equivalents /g) were present in the herbal paste. TLC fingerprints were able to develop for the traditional herbal formulation and its mixture of ingredients.</p> <p><strong>Conclusion: </strong>Present study revealed the presence of phytochemicals such as phenols, flavonoids, tannins and saponins in the traditional herbal preparation.</p> W. M. S. S. K. Kulatunga L. D. A. M. Arawwawala ##submission.copyrightStatement## 2020-01-30 2020-01-30 1 6 Toxicological Evaluation of Different Extracts of Andrographis paniculata <p><strong>Aim:</strong> The study aimed to evaluate the toxicity of the crude extracts of <em>Andrographis paniculata</em> leaves using a Brine Shrimp (<em>Artemia salina</em>) lethality assay test to substantiate the ethnopharmacological uses of this plant.</p> <p><strong>Methodology:</strong> Ethanol, acetone and ethyl acetate extracts of <em>Andrographis paniculata </em>were subjected to qualitative and quantitative phytochemical screening for bioactive compounds using standard methods and protocols. The extracts were further subjected to cytotoxicity screening using Brine Shrimp Lethality Assay.</p> <p><strong>Results:</strong> The results of the preliminary screening revealed the presence of alkaloids, saponins, terpenes, anthraquinones, tannin and flavonoids in all the extracts studied, Phlobatannins and cardiac glycosides were not detected in any of the extracts. Total phenols, alkaloids, flavonoids tannins and saponins were determined quantitatively and the results showed that total phenols were the major phytochemical constituent present in highest concentration while the least was tannin in all extracts. The concentrations of these bioactive compounds were highest in ethanol extract. In Brine Shrimp Lethality Bioassay, all the extracts produced dose-dependent cytotoxicity effect to Brine Shrimp nauplii with ethanol extract exhibiting highest toxicity having LC<sub>50</sub> value of 187.5 μg/ml compared to the positive control, potassium dichromate whose LC<sub>50</sub> value was 130.0 μg/ml.</p> <p><strong>Conclusion:</strong> The cytotoxicity exhibited by the crude extracts confirmed the presence of potent bioactive compounds and validates the ethnopharmacological importance of <em>Andrographis paniculata </em>in the treatment of different illnesses.</p> Imaobong E. Daniel Emmanuel N. Ibok ##submission.copyrightStatement## 2020-02-14 2020-02-14 1 10