Antibiotics Sensitivity Profile of Wounds’ Bacterial Isolates and Antibacterial Assessment of Chromolaena odorata Aqueous and Ethyl Acetate Extracts

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Mercy Adewumi Alabi


Aim: To study the in vitro inhibitory effect of C. odorata extracts on bacterial isolates from wound swab samples.

Place of Study: University of Medical Sciences Teaching Hospital, Akure, Ondo State, Nigeria, between January and June 2019.

Methodology: Wound swabs were collected from patients of University of Medical Sciences Teaching Hospital, Akure. Air-dried and powdered C. odorata leaves were extracted using distilled water and ethyl acetate and concentrated using a rotary evaporator. Qualitative phytochemical screening of C. odorata extracts was done by standard methods. Antibiotics susceptibility pattern of bacterial isolates to a panel of ten (10) conventional antibiotics was determined by disc diffusion method. Antibacterial activity of C. odorata extracts were determined using agar diffusion  method.

Results: Pseudomonas aeruginosa was the most abundant bacteria isolated (40.30%) in this study. Chromolaena odorata aqueous extract had higher extraction yield (24.1%) than ethyl acetate extract. Phytochemical screening revealed the presence of saponin, flavonoids, terpenoids, steroids, tannin, glycoside and phenol of C. odorata extracts. Proteus vulgaris exhibited 100% resistance against augmentin. The highest zone of inhibition observed with the C. odorata ethyl acetate extract was against P. aeruginosa (18.67±0.67 mm).

Conclusion: This study reveals the in vitro inhibitory effect of C. odorata extracts on bacteria isolated from wounds thus presenting them as potential antibacterial agents.

Susceptibility, extraction, inhibitory, phytochemicals, resistance.

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How to Cite
Alabi, M. A. (2019). Antibiotics Sensitivity Profile of Wounds’ Bacterial Isolates and Antibacterial Assessment of Chromolaena odorata Aqueous and Ethyl Acetate Extracts. South Asian Research Journal of Natural Products, 3(`1), 1-9. Retrieved from
Original Research Article


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