Phytochemical Analysis of an Anti-venom Traditional Herbal Preparation for Snake-bite

Main Article Content

W. M. S. S. K. Kulatunga
L. D. A. M. Arawwawala


Objective: Snake-bite is one of the important public health problems of tropical countries including Sri Lanka. The risk of snake-bites is higher in rural areas of the country and people mainly rely on herbal medicines. Antiserum is the only therapeutic agent in Western medicine available throughout the world. A major drawback of serum therapy is its higher cost and also serum sickness is a possible side effect of serum therapy that results in inflammation of tissues and other symptoms. In the present study, an attempt was taken to prepare a traditional herbal paste which used to treat snake-bites and carry out chemical analysis.

Methods: Chemical analysis carried out by investigation of its(a) phytochemical constituents (b) total phenol and flavonoid contents and (c) development of Thin Layer Chromatography (TLC) fingerprints.

Results: Results revealed that phenols, flavonoids, tannins and saponins were abundant in the herbal paste whereas coumarins, terpenoids and alkaloids were absent.  Further high amounts of total phenols (120.30±0.83 mg gallic acid equivalents /g) and flavonoids (69.76±1.62 quercetin equivalents /g) were present in the herbal paste. TLC fingerprints were able to develop for the traditional herbal formulation and its mixture of ingredients.

Conclusion: Present study revealed the presence of phytochemicals such as phenols, flavonoids, tannins and saponins in the traditional herbal preparation.

Chemical constituents, snake –bites, Sri Lankan traditional medicine.

Article Details

How to Cite
S. S. K. Kulatunga, W. M., & A. M. Arawwawala, L. D. (2020). Phytochemical Analysis of an Anti-venom Traditional Herbal Preparation for Snake-bite. South Asian Research Journal of Natural Products, 2(4), 1-6. Retrieved from
Original Research Article


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